蛋白名称：Mouse Legumain/ LGMN Protein (His Tag)
蛋白构建：A DNA sequence encoding the extracellular domain (Val 18-Tyr 435) of mouse LGMN (NP_035305.1) precursor was expressed with a C-terminal polyhistidine tag.
表达宿主：HEK293 Cells
蛋白纯度：≥ 75 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
蛋白活性：Measured by its ability to cleave the fluorogenic peptide substrate, N-carbobenzyloxy-Ala-Ala-Asn-7-amido-4-methyl coumarin(Z-AAN-AMC). The specific activity is > 350 pmoles/min/μg. (Activation description: The enzyme achieves its activity under acidic pH)
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Thr 22
蛋白分子量：The secreted recombinant mouse LGMN consists of 434 amino acids and has a calculated molecular mass of 49.8 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 55 kDa protein in SDS-PAGE under reducing conditions.
蛋白NP号：NP_035305.1
蛋白氨基酸序列：Val18-Tyr435
蛋白标签：N-His
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